《植物生理学报》 2011, 47(6): 551-556

过量表达GLOase导致转基因拟南芥中维生素C含量和抗逆能力提高

李坤岚¹, 郭新波¹, 康以萌¹, 孙小芬¹, 唐克轩^1,2,*

1复旦大学生命科学学院遗传工程国家重点实验室, 复旦-交大-诺丁汉植物生物技术研发中心, 上海200433; 2上海交通大学 农业与生物学院植物生物研究中心, 上海200240

通信作者：唐克轩；E-mail: kxtang1@yahoo.com；Tel: 021-65643552

摘　要：

L-古洛糖酸-1,4-内酯氧化酶(L-gulono-1,4-lactone oxidase, GLOase)是维生素C合成途径中最后一步关键酶, 小鼠(Mus musculus)编码GLOase的gulo基因转化拟南芥(Arabidopsis thaliana)的转基因株系中维生素C含量最高为5.74 µmol•g-1 (FW), 是野生型的3.46倍、转p2301空载体对照的3.19倍。30%聚乙二醇(PEG-6000)模拟干旱胁迫的不同时间梯度中, 幼苗期转基 因拟南芥丙二醛含量低于同样处理下野生型和对照组拟南芥。不同NaCl浓度的盐胁迫下, 转基因拟南芥在子叶期比野生 型、对照组平均根长更长、侧根发育更好; 幼苗期莲座叶长势更好、丙二醛含量更低。结果显示过量表达GLOase的转基 因拟南芥在维生素C含量提高的同时, 抗胁迫能力有所增强。

关键词：维生素C; 拟南芥; L-古洛糖酸-1,4-内酯氧化酶; 干旱胁迫; 盐胁迫

收稿：2010-12-04   修定：2011-02-07

资助：国家重点基础研究发展计划(973)项目(2007CB108805)。

Increase of Vitamin C Level and Stress Tolerance of Transgenic Arabidopsis thaliana Overexpressing GLOase

LI Kun-Lan¹, GUO Xin-Bo¹, KANG Yi-Meng¹, SUN Xiao-Fen¹, TANG Ke-Xuan^1,2,*

¹State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, Fudan University, Shanghai 200433, China; ²Plant Biotechnology Research Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China

Corresponding author: TANG Ke-Xuan; E-mail: kxtang1@yahoo.com; Tel: 021-65643552

Abstract:

L-gulono-1,4-lactone oxidase (GLOase) was the last key enzyme in native vitamin C synthesis. Gulo which encoded GLOase in mice (Mus musculus) was transformed into Arabidopsis thaliana. Transgenic A. thaliana overexpressing GLOase accumulated vitamin C which was 3.46-fold of WT and 3.19-fold of p2301 blank transformed control. During 30% PEG-6000 mocked drought stress, lower malondialdehyde (MDA) content was witnessed in young transgenic A. thaliana plants, which was a demonstration of better anti-drought ability. NaCl salt stress assay showed the enhanced salt tolerance of transgenic A. thaliana plants concerning root development and MDA level. The results indicated that overexpressing GLOase increased vitamin C level and stress tolerance of transgenic A. thaliana plants.

Key words: vitamin C; Arabidopsis thaliana; L-gulono-1,4-lactone oxidase; drought stress; salt stress